Available assays

In addition to the CALUX® assays that are currently available, BDS is constantly working on new reporter cell lines and new applications with these cell lines.

Please find a detailed elaboration on all available assays in our catalogue.

We welcome you to contact Bart van der Burg for further questions and suggestions.

 

Table 1. Overview of HTS CALUX® panel

Name Pathway/Effect Reference Compound Key Reference
DR CALUX® Xenobiotics metabolism / dioxin receptor activation 2,3,7,8-TCDD Aarts et al., 1995
PAH CALUX® Xenobiotics metabolism / dioxin receptor activation benzoapyrene Pieterse et al., 2013
ER CALUX® Estrogen signalling 17β-estradiol Legler et al., 1999
ERα CALUX® Estrogen receptor α-mediated signalling 17β-estradiol Sonneveld et al., 2005; OECD, unpublished
ERα-anti CALUX® Repression of estrogen receptor α-mediated signalling tamoxifen Van der Burg et al., 2010a; OECD, unpublished
ERβ CALUX® Estrogen receptor β-mediated signalling 17β-estradiol Van der Burg et al., 2013
ERβ-anti CALUX® Repression of estrogen receptor β-mediated signalling tamoxifen -
AR CALUX® Androgen receptor activation dihydrotestosterone Sonneveld et al., 2005; OECD, unpublished
AR-anti CALUX® Repression androgen receptor activation flutamide Van der Burg et al., 2010b; OECD, unpublished
PR CALUX® Progesterone receptor-mediated signalling progesterone Sonneveld et al., 2005
PR-anti CALUX® Repression of progesterone receptor-mediated signalling RU486 -
GR CALUX® Glucocorticoid receptor-mediated signalling dexamethasone Sonneveld et al., 2005
anti-GR CALUX® Repression of glucocorticoid receptor-mediated signalling RU486 -
TR CALUX® Thyroid receptor-mediated signalling T3 Piersma et al., 2013; Van der Burg et al., 2013
RAR CALUX® Retinoic acid receptor-mediated signalling retinoic acid Piersma et al., 2013; Van der Burg et al., 2013
PPARγ CALUX® PPARγ-mediated signalling rosiglitazone Gijsbers et al,. 2011
PPARα CALUX® PPARα-mediated signalling GW7674 Gijsbers, submitted
PPARδ CALUX® PPARδ-mediated signalling L165041 Szalowska et al., 2014
LXR CALUX® LXR-mediated signalling GW3965 unpublished
kappaB CALUX® NFκB-mediated signalling TPA Piersma et al., 2013; Van der Burg et al., 2013
P21 CALUX® Transcription of p21 inhibitor of cell cycle progression actinomycin D Piersma et al., 2013; Van der Burg et al., 2013
Nrf2 CALUX® Activation of the Nrf2 pathway / oxidative stress response curcumin Van der Linden et al., 2014
P53 CALUX® p53-dependent pathway activation / genotoxicity response (+/- S9) ( actinomycin D Van der Linden et al., 2014
genotox CALUX® p53-dependent pathway activation +/-S9 cyclophosphamide Van der Linden et al., 2014
TCF CALUX® wnt/TCF pathway activation lithium chloride Piersma et al., 2013; Van der Burg et al., 2013
AP1 CALUX® AP1 pathway activation / cell cycle control TPA Piersma et al., 2013; Van der Burg et al., 2013
Hif1α CALUX® Chemical hypoxia response cobaltous chloride Piersma et al., 2013; Van der Burg et al., 2013
ER stress CALUX® Endoplasmic reticulum stress response tunicamycin Piersma et al., 2013; Van der Burg et al., 2013
cytotox CALUX® Repression of constitutive transcriptional activation Tributyltin acetate Van der Linden et al., 2014