BioDetection Systems organises together with CAAT, TNO, JRC, Biopredic, BASF and DTU a 2-day training for:
Quantitive Human Cell & Effect Based In Vitro Bioanalysis for Assessing Endocrine Disrupting Compound (EDCs)
on 7th-8th September 2017 on the Science Park in Amsterdam
You will learn in a 2 day extensive hands-on training how you can use a panel of standardized stable human- and yeast reporter cell lines for rapid screening of hazards of pure chemicals and complex mixtures on multiple adverse outcome pathways. This includes those pathways prioritized in the current regulatory context of EDC assessment, including interference with estrogen-, androgen-, and thyroid hormone signaling. This will include assessment of metabolic conversions (S9, metabolically competent liver cells) of EDCs using various approaches and assessment of their effects on steroidogenesis.
You will learn more about modern robotic and automated high-through-put screening using down-scaled 96-wells to 384-wells formats. The chemical activated luciferase (CALUX) assays are available for testing of relevant matrices such as food, blood, urine, mother milk and many different environmental matrices.
All steps needed from extraction, clean-up, cell culture handling, luminometer analysis and calculations of effect concentrations and toxic equivalents will be presented in state of the art manner following current guidelines (e.g. ISO 17025, OECD TG455-like, ISO 19040-3).
You will learn to use these data in the context of chemical risk assessment, including in silico modeling, metabolism of compounds, database use and comparative data analysis and read-across, and also linkage to so-called adverse outcome pathways.
Additionally in several oral presentations the current applications in chemicals, mixture toxicity, food, environmental and public health testing are presented.