Identification of bioactive compounds in mixtures using effect-directed analysis

If a desired activity profile is obtained, indicating the presence of bioactive compounds with a positive health effect, as a next step the compound(s) responsible for this bioactivity can be purified through a step-wise fractionation coupled to bioactivity testing of the obtained active mixtures. This effect-directed analysis is continued until a relatively pure biologically active fraction is obtained that can be subjected to chemical analytics to identify the compound(s) of interest. If successful, the CALUX® methods already provide important information on its mode-of-action, while follow-up studies using suitable cell types can complement this knowledge. The identification of the compound, together with the assessment of its mode of action, and if possible combined with historic use of the crude sample can then form the basis of a health claim.

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For further information, please see the relevant references section, or contact Harrie Besselink or Bart van der Burg.